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/^((29([-\/])02\3(\d{2}([13579][26]|[2468][048]|04|08)|(1600|2[048]00)))|((((0[1-9]|1\d|2[0-8])([-\/])(0[1-9]|1[012]))|((29|30)([-\/])(0[13-9]|1[012]))|(31([-\/])(0[13578]|1[02])))(\11|\15|\18)\d{4}))$/

/^((02([-\/])29\3(\d{2}([13579][26]|[2468][048]|04|08)|(1600|2[048]00)))|((((0[1-9]|1[012])([-\/])(0[1-9]|1\d|2[0-8]))|((0[13-9]|1[012])([-\/])(29|30))|((0[13578]|1[02])([-\/])31))(\11|\15|\19)\d{4}))$/

/^(((\d{2}([13579][26]|[2468][048]|04|08)|(1600|2[048]00))([-\/])02(\6)29)|(\d{4}([-\/])((0[1-9]|1[012])(\9)(0[1-9]|1\d|2[0-8])|((0[13-9]|1[012])(\9)(29|30))|((0[13578]|1[02])(\9)31))))$/

/^(((\d{2}([13579][26]|[2468][048]|04|08)|(1600|2[048]00))([-\/])02(\6)29)|(\d{4}([-\/])((0[1-9]|1[012])(\9)(0[1-9]|1\d|2[0-8])|((0[13-9]|1[012])(\9)(29|30))|((0[13578]|1[02])(\9)31)))) (\d|[0-1]\d|[2][0-3]):[0-5]\d$/

/^(?!\.)((?!.*\.{2})[a-zA-Z0-9\u0080-\u02AF\u0300-\u07FF\u0900-\u18AF\u1900-\u1A1F\u1B00-\u1B7F\u1D00-\u1FFF\u20D0-\u214F\u2C00-\u2DDF\u2F00-\u2FDF\u2FF0-\u2FFF\u3040-\u319F\u31C0-\uA4CF\uA700-\uA71F\uA800-\uA82F\uA840-\uA87F\uAC00-\uD7AF\uF900-\uFAFF!#$%&'*+\-/=?^_`{|}~\d]+)(\.[a-zA-Z0-9\u0080-\u02AF\u0300-\u07FF\u0900-\u18AF\u1900-\u1A1F\u1B00-\u1B7F\u1D00-\u1FFF\u20D0-\u214F\u2C00-\u2DDF\u2F00-\u2FDF\u2FF0-\u2FFF\u3040-\u319F\u31C0-\uA4CF\uA700-\uA71F\uA800-\uA82F\uA840-\uA87F\uAC00-\uD7AF\uF900-\uFAFF!#$%&'*+\-/=?^_`{|}~\d]+)*@(?!\.)([a-zA-Z0-9\u0080-\u02AF\u0300-\u07FF\u0900-\u18AF\u1900-\u1A1F\u1B00-\u1B7F\u1D00-\u1FFF\u20D0-\u214F\u2C00-\u2DDF\u2F00-\u2FDF\u2FF0-\u2FFF\u3040-\u319F\u31C0-\uA4CF\uA700-\uA71F\uA800-\uA82F\uA840-\uA87F\uAC00-\uD7AF\uF900-\uFAFF\-\.\d]+)((\.([a-zA-Z\u0080-\u02AF\u0300-\u07FF\u0900-\u18AF\u1900-\u1A1F\u1B00-\u1B7F\u1D00-\u1FFF\u20D0-\u214F\u2C00-\u2DDF\u2F00-\u2FDF\u2FF0-\u2FFF\u3040-\u319F\u31C0-\uA4CF\uA700-\uA71F\uA800-\uA82F\uA840-\uA87F\uAC00-\uD7AF\uF900-\uFAFF]){2,63})+)$/i

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HUG2hm7Hk6pid9d2AgVIMu6APBR7JRu6LQ2PBnhoHbKAQMMhT6HPt2IEXKLzZMZQCQqGzGsazwVRpnq6tWtY8I58IJYKo5Yk9qADAJRtwyK6epupg5gteqQh

iPSC & Fibroblast Information Collection Form

Thank you for considering to share samples with NCRAD. Your contribution is an important addition to the research community. As an added benefit to sharing your samples with NCRAD, the quality control results will be returned to you once your lines have been processed by the IUGB.

Please complete the survey below to provide information about the samples you plan to share. Thank you!

Note: We request that investigators submit 3 vials per iPSC and fibroblast line. This ensures the lab has extra vials in the event of a QC measure failure. Sending less than 3 vials could result in additional requests for any vials that fail QC.

Note: If you are depositing fibroblast-derived iPSCs, please send original fibroblast lines in the same shipment.

Yes

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iPSCs

Fibroblasts

1-5

6-10

11-15

16-20

21-25

26-30

31-35

36-40

41-45

46-50

51+

reset

Wild Type

Mutation

Sporadic AD

APOE4

APP

c9orf72

MAPT

PGRN

PSEN1

PSEN2

TREM2

Other

Patient-derived

Genetically engineered

Yes

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iPSC - Please deposit any isogenic lines, if available.

Yes

reset

mTesr1

Tesr2

mTesr E8

mTesr 3D

Nutristem

StemMACS iPS-Brew XF

Unknown

Other

Matrigel

Vitronectin

Laminin

None

Unknown

Other

DMSO

Cryostore

mFreSR

Gibco PSC Cryopreservation

Synth-a-Freeze

Unknown

Other

1-15

16-20

21-25

26-30

31+

Unknown

< 500,000

500,000-1,000,000

>1,000,000

Unknown

Episomal

mRNA

Retroviral

Lentiviral

Sendai Virus

Unknown

Other

Dispase

Collagenase

Accutase

EDTA

Unknown

Other

5% CO2

Unknown

Other

Atmospheric

Hypoxic

Unknown

Mycoplasma free

Not tested/unknown

Upload file

1-5

6-10

11-15

16-20

21-25

26-30

31+

Yes

Yes

Unknown

reset

Expand

1-5

6-10

11-15

16-20

21-25

26-30

31-35

36-40

41-45

46-50

51+

reset

Wild Type

Mutation

APOE4

APP

c9orf72

MAPT

PGRN

PSEN1

PSEN2

TREM2

Other

RPMI 1640 + FBS

DMEM + FBS

DMEM/F12 + FBS

Unknown

Other

growth medium + DMSO

growth medium + glycerol

Unknown

Other

1-5

6-10

11-15

16-20

21-25

26+

Unknown

reset

1 to 9

10 to 19

20 to 29

30 to 39

40 to 49

50+

Unknown

reset

< 500,000

500,000-1,000,000

>1,000,000

Unknown

Trypsin EDTA

Unknown

Other

5% CO2

Unknown

Other

Atmospheric

Hypoxic

Unknown

Mycoplasma free

Not tested/unknown

Upload file

1-5

6-10

11-15

16-20

21-25

26-30

31+

Yes

Yes

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